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Cytoplasmic genetic male-sterility is used to produce hybrid onion (Allium cepa L.) seeds worldwide. In this paper, we present the results of research aimed toward identifying PCR-based markers linked to the Ms locus through amplified fragment length polymorphism (AFLP). After screening 512 AFLP primer combinations, only one AFLP fragment was identified as(More)
Selection for maintainer lines using molecular markers that distinguish genotypes at the male restorer-of-fertility (Ms) locus would improve the efficiency of breeding F1 hybrids. Here, we developed a multiplex PCR-based co-dominant marker (AcSKP1) in complete linkage disequilibrium with the Ms locus in onion through allele mining and demonstrated its(More)
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