Steven W. Mitchell

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By means of a selective DNA amplification technique called polymerase chain reaction, proviral sequences of the human immunodeficiency virus (HIV-1) were identified directly in DNA isolated from peripheral blood mononuclear cells (PBMCs) of persons seropositive but not in DNA isolated from PBMCs of persons seronegative for the virus. Primer pairs from(More)
Efforts to solve the epidemiologic puzzle of AIDS in Africa are complicated by the presence of multiple human retroviruses. Simple serologic tests that unambiguously distinguish among infections by these retroviruses are essential. To that end, a partially conserved immunoreactive epitope was identified in the transmembrane glycoproteins of human(More)
The in-vitro effect of ribavirin on the replication of lymphadenopathy-associated virus (LAV), one of the prototype viruses aetiologically associated with lymphadenopathy syndrome and acquired immunodeficiency syndrome, was tested. Ribavirin, a nucleoside, suppressed the replication of LAV in cultures of human adult T lymphocytes. Suppression occurred at(More)
The presence of antibodies to lymphadenopathy-associated retrovirus (LAV) was determined by a radioimmunoprecipitation assay and by an enzyme-linked immunosorbent solid assay of sera from Zairian patients with the acquired immune deficiency syndrome (AIDS) in 1983. Thirty-five of 37 patients (94 percent) and 32 of 36 patients (88 percent), respectively,(More)
1-Efficient management of the product configuration process is a challenge in the evolution of any industrial scale product family. This is particularly true with current standards-based system modeling tools, as the standards themselves are just beginning to address this problem in a scalable fashion. In the case of the SWFTS common submarine combat(More)
Managing the evolution of a complex product family that is deployed and maintained in multiple variants on various platforms using traditional systems engineering tools and processes is a significant challenge. An example is managing the evolution of a common combat system across a fleet of submarines. Due to the realities of budgets and operational(More)
A rapid immunobinding assay ('HIVCHEK', Ortho) and an agglutination assay ('Serodia-HIV', Fujirebio) were evaluated as an alternative to enzyme-linked immunosorbent assay (ELISA) and western blot under field conditions in Africa for detection of antibody to human immunodeficiency virus (HIV). 7106 specimens were tested at 25 laboratories in Kenya, Ghana,(More)