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RNAi-Mediated Targeting of Heterochromatin by the RITS Complex
RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termedExpand
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Differential regulation of EGF receptor internalization and degradation by multiubiquitination within the kinase domain.
Ubiquitination of the EGF receptor (EGFR) is believed to play a critical role in regulating both its localization and its stability. To elucidate the role of EGFR ubiquitination, tandem massExpand
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Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
IFN-alpha/beta plays an essential role in innate immunity against viral and bacterial infection. Among the proteins induced by IFN-alpha/beta are the ubiquitin-like ISG15 protein and its E1- (Ube1L)Expand
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Target-Decoy Search Strategy for Mass Spectrometry-Based Proteomics
Accurate and precise methods for estimating incorrect peptide and protein identifications are crucial for effective large-scale proteome analyses by tandem mass spectrometry. The target-decoy searchExpand
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A Conserved MST-FOXO Signaling Pathway Mediates Oxidative-Stress Responses and Extends Life Span
Oxidative stress influences cell survival and homeostasis, but the mechanisms underlying the biological effects of oxidative stress remain to be elucidated. Here, we demonstrate that the proteinExpand
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The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice.
Nearly every extracellular ligand that has been found to play a role in regulating bone biology acts, at least in part, through MAPK pathways. Nevertheless, much remains to be learned about theExpand
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Multiple mechanisms collectively regulate clathrin-mediated endocytosis of the epidermal growth factor receptor
Four independent mechanisms for uptake of activated EGFR are identified by a combination of receptor mutagenesis and RNA interference approaches.
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Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex
Cohesin is a multi-subunit, ring-shaped protein complex that holds sister chromatids together from the time of their synthesis in S phase until they are segregated in anaphase. In yeast, the loadingExpand
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SKAR Is a Specific Target of S6 Kinase 1 in Cell Growth Control
BACKGROUND The mammalian target of rapamycin (mTOR) and phosphatidylinositol 3-kinase (PI3K) signaling pathways promote cell growth and cell cycle progression in response to nutritional, energy, andExpand
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Tome-1, a Trigger of Mitotic Entry, Is Degraded during G1 via the APC
Entry into mitosis requires the activation of cdk1/cyclin B, while mitotic exit is achieved when the same kinase activity decreases, as cyclin B is degraded. Cyclin B proteolysis is mediated by theExpand
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