Steven D. Schimmel

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Partially purified plasma membranes were obtained from chick-embryo muscle cells grown in tissue culture. The purification procedure involved homogenization in buffered isotonic sucrose followed by differential and sucrose density gradient centrifugations. The activities of five plasma-membrane markers, as well as microsomal and mitochondrial markers, were(More)
We have studied the development of high affinity insulin receptors and insulin-stimulated responses in the differentiating nonfusing muscle cell line BC3H-1. In the logarithmic growth phase, these myoblasts exhibit very low levels of insulin binding and no detectable insulin-stimulated glucose or amino acid uptake. Following the cessation of cell division(More)
Ca++-dependent degradation of triphosphoinositide has been postulated to regulate levels of membrane-bound Ca++ and to generate a 1,2-diacylglycerol fusogen in cell fusion. Triphosphoinositide metabolism was therefore studied during Ca++-induced fusion of cultured chick embryo myoblasts. Using a frequently cited extraction procedure, it was found that(More)
We recently reported that treatment of differentiated chick embryo myoblasts in culture with the potent tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) caused a 2-fold increase in the level of 1,2-diacylglycerol in the plasma membrane fraction within 15-30 min (Grove, R.I. and Schimmel, S.D. (1981) Biochem. Biophys. Res. Commun. 102, 158-164).(More)