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We investigated properties of the rotavirus genome segment 11 protein. A rotavirus SA11 genome segment 11 cDNA which contains the entire coding region was sequenced and inserted into the baculovirus transfer vector pVL941. Recombinants containing gene 11 cDNA were selected, and the gene 11 product expressed in Spodoptera frugiperda cells infected with these(More)
Paired nasopharyngeal and oropharyngeal swabs collected from 533 children hospitalized with lower respiratory tract infection were assessed by multiplex reverse transcription-PCR. Oropharyngeal swabs increased the number of viral infections detected by 15%, compared to collection of a nasopharyngeal swab alone. This advantage was most pronounced for(More)
A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the(More)
Detection of respiratory viruses by real-time multiplexed PCR (M-PCR) and of respiratory syncytial virus (RSV) by M-PCR and immunofluorescence (IF) was evaluated using specimens collected by nasopharyngeal flocked swabbing (NFS) and nasal washes (NW). In children with mild respiratory illness, NFS collection was superior to NW collection for detection of(More)
Three monoclonal antibodies (MAbs)--B5C9, B5E4, and B10G10--to human group B rotavirus, an agent implicated in epidemic outbreaks of diarrhea in the People's Republic of China, primarily in adults, were prepared. MAb reactivity was decreased when virus preparations were treated with EDTA, suggesting reactivity with the outer-capsid protein(s). Competition(More)
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We present an innovative backup and restore solution, called LifeBoat, for Windows machines. Our solution provides for local and remote backups and ''bare metal'' restores. Classic backup systems do a file system backup and require the machine to be installed before the system can be restored or they do a block for block backup of the system image which(More)
We have developed a prototype for sentiment analysis that is able to identify aspects of an entity being reviewed, along with the sentiment polarity associated to those aspects. Our approach relies on a core ontology of the task, augmented by a workbench for bootstrap-ping, expanding and maintaining semantic assets that are useful for a number of text(More)
We have developed an application that helps users to tailor a message to a particular audience. It uses a message resonance model that rates the wording of a message based on an analysis of millions of pieces of data with regard to their historical success in the context of a target audience. We have shown that higher resonance messages are twice as likely(More)