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We have constructed a Xenopus laevis oocyte expression vector, pOEV, which allows cloned DNA to be transcribed and translated directly in the oocyte. Since proteins translated in oocytes are post-translationally modified according to conserved eukaryotic signals, these cells offer a convenient system for performing structural and functional analyses of(More)
The phenomenon of the wandering point on a blank sheet of paper in serial reproductions is the starting point of an investigation of the perceptual field structure of homogeneous stimulus areas; 609 stimulus points distributed regularly in 21 rows and 29 columns on a DIN A4 sheet were presented successively to 10 subjects and had to be reproduced(More)
TFIIIA mRNA expression is developmentally regulated during Xenopus oogenesis. We have identified a positive-acting cis-element in the TFIIIA gene located between -671 and -629 with respect to the mRNA initiation site, termed element 3. Element 3 contributes to the efficient transcription of the TFIIIA gene in stage III oocytes, but is inactive in the normal(More)
Perceptual multistability refers to cases where perception alternates between two or more interpretations of an unchanging sensory stimulus. In a first experiment we trained eight pigeons to discriminate horizontal and vertical apparent motion stimuli and then presented a multistable display. In five cases their behavior showed alternations similar to human(More)
The Xenopus TFIIIA gene is transcribed very efficiently in oocytes. In addition to a TATA element at -30, we show that from -425 to +7 the TFIIIA gene contains only two positive cis elements centered at -267 (element 1) and -230 (element 2). This arrangement of the cis elements in the TFIIIA gene is striking because these two elements are positioned very(More)
Expression of the Xenopus laevis transcription factor IIIA (TFIIIA) gene is developmentally regulated. In this study we have used defined nucleotide mutations to map cis-elements involved in transcriptional regulation of the promoter for oocyte-TFIIIA (O-TFIIIA) in stage II-IV oocytes, stage VI oocytes, and tail bud embryos. During oogenesis O-TFIIIA mRNA(More)
In recent years, several prominent political scientists have argued that quantitative and qualitative methodologies should be seen as united by a single logic of scientific inference. King, Keohane and Verba’s seminal book Designing Social Inquiry ~1994, hereafter referred to as KKV!, which jumpstarted this debate, emphasized how scholars might transfer(More)
The transforming (onc) genes of retroviruses contain specific sequences, derived from as yet poorly defined, normal cellular genes, termed proto-onc genes. Proto-onc genes must be defined to explain their docility compared to the oncogenicity of the viral derivatives. Here we set out to determine the borders of the chicken proto-fps gene from which the onc(More)
The oncogenic avian retrovirus OK10 has the genetic structure gag-delta pol-myc-delta-env. The myc sequence is transduced from a cellular gene, proto-myc, while gag, pol, and env are essential retrovirus genes. By analogy with other directly oncogenic retroviruses, the specific myc sequence of OK10 is thought to be essential for transforming function.(More)
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