Stephen R. Watson

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An in vitro construct of human skin (living skin equivalent, LSE) has been engineered using serially passaged human epidermal keratinocytes and human dermal fibroblasts with a matrix of type I collagen. Cells are obtained from neonatal foreskin. LSE is cast, cultured, and shipped in a single culture insert. The size and shape of the insert determines the(More)
The technology for culture of epidermis is one of the most advanced to date for generation of a tissue in vitro. Cultured epidermis is already used for a number of applications ranging from use as a permanent skin replacement to use as an organotypic culture model for toxicity testing and basic research. While simple epidermal sheets have been grafted(More)
In order to perform their role as mediators of inflammation neutrophils must be able to extravasate from the blood into the surrounding tissue. The initial step in this process is the rolling of neutrophils along the endothelium and is mediated by adhesion molecules known as selectins. This review will discuss the in vitro and in vivo characterization of an(More)
Risk is the focal topic in the management of many activities and technologies. For that management to be successful, an explicit and accepted definition of the term "risk" is essential. Creation of that definition is a political act, expressing the definers' values regarding the relative importance of different possible adverse consequences for a particular(More)
Microfinance has over three decades of experience serving the needs of the poor on earth. Unfortunately, academia and the secular world are divided on its effectiveness to lift the impoverished from the bottom of the economic pyramid. India is the focus of this paper, but measures of microfinance success are essentially universal. This paper examines the(More)
We have shown previously that spleen cells from mice pre-treated with C. parvum give enhanced antibody response when cultured in the presence of thymus-dependent antigens. In order to establish if C. parvum has a similar effect when added in vitro, spleen cells from untreated mice were cultured with C. parvum in Marbrook chambers with sheep erythrocytes(More)
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