In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that… (More)
This is the eBook version of the printed book. What happens when you've built a great website or app, but no one seems to care? How do you get people to stick around long enough to see how your service might be of value? In Seductive Interaction Design, speaker and author Stephen P. Anderson takes a fresh approach to designing sites and interactions based… (More)
Chair Fine welcomed members of the UCAR Board of Trustees, representatives from the Member institutions, representatives from the Academic Affiliates, members of the President's Advisory Committee on University Relations, NSF sponsors, and early career faculty.
Opaque interactions have been recognised as a challenge for Optimality Theory (OT) for a long time. We show that although there has been considerable effort to bring opacity into the scope of OT, some types of process interactions are still problematic for the theory. Based on data from Tundra Nenets, we present and analyse a case of fed counterfeeding in… (More)
Antibodies are key reagents in biology and medicine, but commercial sources are rarely recombinant and thus do not provide a permanent and renewable resource. Here, we describe an industrialized platform to generate antigens and validated recombinant antibodies for 346 transcription factors (TFs) and 211 epigenetic antigens. We describe an optimized… (More)
Studies using event-related brain potentials (ERPs) to examine feedback processing in gambling tasks have focused almost exclusively on components elicited between 200 and 500ms after feedback over the frontal-central region of the scalp (i.e., P2, feedback negativity (FN), and P3a). In contrast, studies examining the functional neuroanatomy of feedback… (More)
BACKGROUND To isolate over-secretors, we subjected to saturation mutagenesis, a strain of P.pastoris exporting E. coli alkaline phosphatase (EAP) fused to the secretory domain of the yeast α factor pheromone through cellular PHO1/KEX2 secretory processing signals as the α-sec-EAP reporter protein. Direct chromogenic staining for α-sec-EAP activity is… (More)
The Center for Applied Research in Human Development is a joint venture between the department of Human Development and Family Studies, in the College of Liberal Arts and Sciences, and Cooperative Extension System in the College of Agriculture and Natural Resources. The Center provides assistance to state and community-based agencies in the development,… (More)
No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form (electronic or otherwise) without prior permission from firstname.lastname@example.org. EDITORIALS 799 Where are the data? 799 And are the tools available?
We examined age-related differences in the spatiotemporal distribution of event-related brain potentials (ERPs) related to feedback processing in a virtual blackjack game. The behavioral data revealed that older adults were less risk seeking than younger adults both within and across trials. Age-related differences were observed in the amplitude of several… (More)