Stephen Fields

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Sodium-dependent neurotransmitter transporters participate in the clearance and/or recycling of neurotransmitters from synaptic clefts. The snf-11 gene in Caenorhabditis elegans encodes a protein of high similarity to mammalian GABA transporters (GATs). We show here that snf-11 encodes a functional GABA transporter; SNF-11-mediated GABA transport is Na+ and(More)
—Facial composites are pictures of human faces. These are normally constructed by victims and witnesses of crime who describe a suspect's face and then select individual facial features. Unfortunately, research has shown that composites constructed in this way are not often recognised. In contrast, we are quite good at recognizing complete faces, even if(More)
The neurotransmitter acetylcholine (ACh) is specifically synthesized by the enzyme choline acetyltransferase (ChAT). Subsequently, it is loaded into synaptic vesicles by a specific vesicular acetylcholine transporter (VAChT). We have generated antibodies that recognize ChAT or VAChT in a model organism, the nematode Caenorhabditis elegans, in order to(More)
Some unicellular organisms present challenges to chemical fixations that lead to common, yet obvious, artifacts. These can be avoided in entirety by adapting spray-freezing technology to ultrarapidly freeze specimens for freeze substitution. To freeze specimens, concentrated suspensions of cells ranging in diameter from 0.5-30 pm were sprayed with an(More)
Introduction Artificial insemination provides a method to inseminate a large number of females to a single sire selected and proven to be an industry leader for economically relevant traits. Thus, genetic change in a herd can occur quickly through the use of artificial insemination. With natural service, herd bulls are also selected for economically(More)
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