Stephanie Sisk

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Chronic exposure to 1,3-butadiene (BD) results in early occurrence and high incidence of lethal lymphomas in male B6C3F1 mice. Male B6C3F1 lacI transgenic mice (BigBlue) were exposed by inhalation to 0, 62.5, 625 or 1250 p.p.m. BD for 4 weeks (6 h/day, 5 days/week). The lacI- mutant frequency and mutational spectrum were evaluated in DNA isolated from bone(More)
We are using B6C3F1 lacI transgenic mice to investigate the in vivo mutagenicity and mutational spectra of 1,3-butadiene (BD). Male B6C3F1 lacI transgenic mice were exposed by inhalation to 0, 62.5, 625, or 1250 ppm BD for 4 weeks (6 h/day, 5 days/week). Tissues were collected 2 weeks after the final exposure for mutant frequency analysis. The lacI- mutant(More)
Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of > or = 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of(More)
We have examined the mutagenicity of 1,3-butadiene in vivo in transgenic mice constructed with lambda phage shuttle vectors that contain either lacZ or lacI as transgenes. Thus far, we have shown that butadiene is mutagenic to the lung of lacZ mice (CD2F1, 625 ppm butadiene, 6 h/day, 5 days) and to the bone marrow of lacI mice (B6C3F1, 62.5, 625, 1250 ppm(More)
In the present study, the lacI mutant frequency was determined in the tissues of B6C3F1 lacI transgenic mice exposed by inhalation to ethylene oxide (EO). Groups of 15 male transgenic lacI B6C3F1 mice were exposed to either 0, 50, 100, or 200 ppm EO for 4 weeks (6 h/day, 5 days/week) and were sacrificed at 0, 2, or 8 weeks after the last EO exposure. The(More)
Ethylene oxide (EO) is a direct-acting alkylating agent with the potential to induce cytogenetic alterations, mutations, and cancer. In the present study, the in vivo mutagenicity of EO at the hypoxanthine guanine phosphoribosyltransferase (hprt) locus of T-lymphocytes was evaluated following inhalation exposure of male B6C3F1 lacI transgenic mice. For this(More)
Three murine liver glutathione transferase (GT, EC have been cloned and sequenced. Two of the cDNA clones, pGT875 and pGT55, encode the murine class-mu GT isoenzymes, GT8.7 and GT9.3, respectively. These two cDNA clones share 85% DNA sequence identity with one another, and the GT8.7 sub-unit encoded by pGT875 shares 92% protein sequence identity(More)
Levels of mRNAs encoding class-alpha glutathione transferases, class-mu glutathione transferases, quinone reductase, and cytochrome P450 1A were measured after xenobiotic induction in murine tissues and in the Hepa1c1c7 murine hepatoma cell line. RNA levels in liver and intestinal mucosa were determined after induction with phenobarbital, butylated(More)