Steffen Wolf

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OBJECTIVE Critical illness polyneuropathy/myopathy (CIP/CIM) is frequently described in critically ill patients who survive severe sepsis. Clinically relevant paresis is major symptom of CIP/CIM. We aimed at determining risk factors and diagnostic value of electrophysiologic testing for CIP/CIM in patients with acute respiratory distress syndrome (ARDS). (More)
Accessory β-subunits of the KCNE gene family modulate the function of various cation channel α-subunits by the formation of heteromultimers. Among the most dramatic changes of biophysical properties of a voltage-gated channel by KCNEs are the effects of KCNE1 on KCNQ1 channels. KCNQ1 and KCNE1 are believed to form nativeI(Ks) channels. Here, we characterize(More)
The mammalian olfactory system comprises a large family of G protein-coupled receptors (GPCRs) to detect and discriminate numerous volatile ligands. More than 350 human genes encode functional olfactory receptors (ORs) that belong to the class A (rhodopsin-like) GPCR family. Owing to difficulties with functional OR expression in heterologous systems, only a(More)
Protein-bound internal water molecules are essential features of the structure and function of microbial rhodopsins. Besides structural stabilization, they act as proton conductors and even proton storage sites. Currently, the most understood model system exhibiting such features is bacteriorhodopsin (bR). During the last 20 years, the importance of water(More)
The light-activated microbial ion channel channelrhodopsin-2 (ChR2) is a powerful tool to study cellular processes with high spatiotemporal resolution in the emerging field of optogenetics. To customize the channel properties for optogenetic experiments, a detailed understanding of its molecular reaction mechanism is essential. Here, Glu-90, a key residue(More)
Objectives: To study the effects of an inhaled endothelin A (ETA) receptor antagonist on hemodynamics and pulmonary gas exchange in experimental acute lung injury (ALI). Design and setting: Prospective, randomized, and controlled study in a university laboratory. Participants and interventions: Sixteen pigs were ventilated in a volume controlled mode during(More)
We studied the effects of the inhaled endothelin-A receptor antagonist LU-135252 at different doses on hemodynamics and gas exchange in an animal model of acute lung injury. Thirtysix piglets (27 +/- 1 kg) were anesthetized, mechanically ventilated (FiO2 1.0), and surfactant-depleted by repeated lung lavage. The animals were randomly assigned to receive(More)
A unique RNA-RNA interaction occurs between uncharged tRNA and the untranslated mRNA leader region of bacterial T box genes. The interaction results in activation of a transcriptional antitermination molecular switch (riboswitch) by stabilizing an antiterminator RNA element and precluding formation of a competing transcriptional terminator RNA element. The(More)
OBJECTIVES Comparison of one-plane and two-plane external fixation in terms of successful healing, incidence of complications, and biomechanical stability in a sheep model. BACKGROUND Rigid fixation is preferred in open and comminuted fractures with a reduced blood supply, preventing infection and healing delay, but more often a flexible device is(More)
High-resolution protein ground-state structures of proton pumps and channels have revealed internal protein-bound water molecules. Their possible active involvement in protein function has recently come into focus. An illustration of the formation of a protonated protein-bound water cluster that is actively involved in proton transfer was described for the(More)