Learn More
The clear importance of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) as an in-vitro model highlights the relevance of studying these cells and their function also in-silico. Moreover, the phenotypical differences between the hiPSC-CM and adult myocyte action potentials (APs) call for understanding of how hiPSC-CMs are maturing(More)
RATIONALE Sodium channel blockers are used as gene-specific treatments in long-QT syndrome type 3, which is caused by mutations in the sodium channel gene (SCN5A). Response to treatment is influenced by biophysical properties of mutations. OBJECTIVE We sought to investigate the unexpected deleterious effect of mexiletine in a mutation combining gain-of-(More)
We survey the state-of-the-art on the Internet-of-Things (IoT) from a wireless communications point of view, as a result of the European FP7 project BUTLER which has its focus on pervasiveness, context-awareness and security for IoT. In particular, we describe the efforts to develop so-called (wireless) enabling technologies, aimed at circumventing the many(More)
AIMS Polyunsaturated fatty n-3 acids (PUFAs) have been reported to exhibit antiarrhythmic properties. However, the mechanisms of action remain unclear. We studied the electrophysiological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on IKs, and on the expression and location of Kv7.1 and KCNE1. METHODS AND RESULTS Experiments were(More)
BACKGROUND Alterations of ventricular repolarization duration, as measured by the QT interval, are frequently observed in haemodialysis (HD) patients. The nature and the sign of these changes are not yet fully understood. METHODS Different dialysate K(+) and Ca(2+) levels, leading to different end-HD plasma concentrations in the patient, have been tested(More)
Reverse rate dependence is a problematic property of antiarrhythmic drugs that prolong the cardiac action potential (AP). The prolongation caused by reverse rate dependent agents is greater at slow heart rates, resulting in both reduced arrhythmia suppression at fast rates and increased arrhythmia risk at slow rates. The opposite property, forward rate(More)
The human embryonic stem cell (hESC) line H1 from WICELL RESEARCH INSTITUTE (Madison, WI) was cultivated following WICELL protocols. Briefly, undifferentiated hESCs were grown on irradiated mouse embryonic fibroblasts and passaged once a week using collagenase and mechanical dissociation. Propagation medium was composed of DMEM/F12 supplemented with 20%(More)
The production of disease-specific lines of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) opened new opportunities to study genetic cardiac disorders such as Long QT (LQT) syndrome. We focused on the computational modelling of hiPSC-CMs with LQT1 syndrome which reduces the slow delayed rectifYing, hs, current. Both control and(More)
Inactivation of L-type Ca channels (LTCC) is regulated by both Ca and voltage-dependent processes (CDI and VDI). To differentiate VDI and CDI, several experimental and theoretical studies have considered the inactivation of Ba current through LTCC (I(Ba)) as a measure of VDI. However, there is evidence that Ba can weakly mimic Ca, such that I(Ba)(More)