Stefano Pagliara

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We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations(More)
Organic materials have revolutionized optoelectronics by their processability, flexibility and low cost, with application to light-emitting devices for full-colour screens, solar cells and lasers. Some low-dimensional organic semiconductor structures exhibit properties resembling those of inorganics, such as polarized emission and enhanced(More)
The selectivity and speed of many biological transport processes transpire from a 'reduction of dimensionality' that confines diffusion to one or two dimensions instead of three. This behaviour remains highly sought after on polymeric surfaces as a means to expedite diffusional search processes in molecular engineered systems. Here, we have reconstituted(More)
Although cellular mechanical properties are known to alter during stem cell differentiation, understanding of the functional relevance of such alterations is incomplete. Here, we show that during the course of differentiation of human myeloid precursor cells into three different lineages, the cells alter their viscoelastic properties, measured using an(More)
Silica fi lms are widely used in many fi elds, such as coatings and functionalization layers for biomedical surfaces and tissue engineering, controlled drug delivery, transplants, cell adhesion, growth, and controlled differentiation. [ 1–6 ] Other applications include masters and moulds for soft and nanoimprint lithographies, [ 7 ] diagnostics, optics and(More)
Embryonic stem cells (ESCs) self-renew in a state of naïve pluripotency in which they are competent to generate all somatic cells. It has been hypothesized that, before irreversibly committing, ESCs pass through at least one metastable transition state. This transition would represent a gateway for differentiation and reprogramming of somatic cells. Here,(More)
We introduce the integration of organic, polarised light-emitting electrospun nanofibers and lab-on-a-chip microchannel geometries. The alignment and spinning electric field leads to ordered mesoscopic active areas, up to many mm(2), which exhibit polarised light emission and are fully compatible with microlithographies and microfluidics. We utilise the(More)
Position x P1 P2 P3 Transport of ions, [ 1 , 2 ] proteins, [ 3 ] antibiotics [ 4 , 5 ] and other macromolecular solutes through channels and pores is ubiquitous in nature. In particular channel-facilitated diffusion relies on optimized binding sites for the transported particles inside the channel. [ 6 ] Well characterized examples include membrane channels(More)
We report on the experimental evidence of tilted polymer nanofiber rotation, using a highly focused linear polarized Gaussian beam. Torque is controlled by varying trapping power or fiber tilt angle. This suggests an alternative strategy to previously reported approaches for the rotation of nano-objects, to test fundamental theoretical aspects. We compare(More)
The properties of polymeric nanofibers can be tailored and enhanced by properly managing the structure of the polymer molecules at the nanoscale. Although electrospun polymer fibers are increasingly exploited in many technological applications, their internal nanostructure, determining their improved physical properties, is still poorly investigated and(More)