Stefania Rigotti

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A one-step triplex RT-PCR method was characterised that allows rapid, strain-specific detection of potato virus Y (PVY) occurring on potato: PVY(N), PVY(O), PVY(NTN) (recombinant isolates), PVY(N)Wi and PVY(C). Three specific primer pairs were designed on aligned PVY sequences available from genomic data banks. The specificity of the selected primers was(More)
Random amplified polymorphic DNA (RAPD) assays were applied on 34 fungal strains isolated from strawberry and other host plants, in order to detect polymorphism to consequently identify and isolate molecular markers specific to Botrytis cinerea. Among the 26 10-mer primers tested, one primer mainly amplified a 750-bp product present in all the B. cinerea(More)
The status of the Potato virus Y (PVY) in Swiss seed potato production was investigated in the years 2003 and 2008 by analysing 385 leaf samples of field-grown, suspicious potato plants collected in four representative seed control fields. Serological investigations by ELISA showed that in c. 84% of the PVY-positive samples in both years, viruses belonging(More)
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