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Sapropelic ciliates from anoxic mud samples were enriched and cultivated in monoculture together with natural food bacteria growing on cellulose. The ciliates lacked cytochrome oxidase and contained bluish fluorescent endosymbionts. One of the anaerobic ciliates, Trimyema compressum, contained methanogenic bacteria as was shown by methane formation. During(More)
Anaerobic biodegradation and inhibitory effects of nonionic and anionic surfactants on methanogenic fermentation were tested in incubation experiments with anoxic sediment samples and sewage sludge. Alkylsulfonates and alkylbenzenesulfonates were not degraded but inhibited meth-anogenesis from sludge constituents at concentrations 1> 10 mg l-k Sodium(More)
Two strains of the anaerobic ciliate Trimyema compressum, isolated from different habitats, were compared. The cytoplasm of the ciliates contained hydrogenosome-like microbodies and methanogenic bacteria; the latter were lost during continued cultivation. In addition both strains harbored a non-methanogenic endosymbiont, which was lost in strain K. The(More)
Monoxenic cultures of the anaerobic, endosymbiont-free ciliate Trimyema compressum were incubated with low numbers of Bacteroides sp. strain WoCb15 as food bacteria and two strains (DSM 3636 and 3637) of Methanobacterium formicicum, which originally had been isolated from the anaerobic protozoa Metopus striatus and Pelomyxapalustris. The ciliate which had(More)
OBJECTIVES The point of departure for the present work is the observation that, in comparison with Anglo-American countries or the Netherlands, Germany was responsible for only a marginal number of international publications in the field of medical education research before 2004. Recent years, however, have seen an increase in the importance of medical(More)
INTRODUCTION Progress tests provide students feedback on their level of proficiency over the course of their medical studies. Peer-assisted learning and competency-based education have become increasingly important in medical education. Although progress tests have been proven to be useful as a longitudinal feedback instrument, there are currently no(More)
Emergency response plans are critical for public and occupational safety. " A Novel Approach for Development and Implementation of an Emergency Response Plan for the BSL-3 Laboratory Service in Singapore " (pp. 158-163) provides a blueprint for coordinating the effort of government agencies, public services, and laboratory staff in the prevention and(More)
To generate cloned macrophage populations with sensitivity towards granulocyte/macrophage colony-stimulating factor (GM-CSF), bone marrow-derived macrophages (BMMΦ) were immortalized by transformation with SV40. A panel of transformed clones was established. The majority of clones represented independently derived transformants, as evidenced by restriction(More)
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