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Biochemical characterization of human and murine isoforms of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE)
TLDR
HGNE2, recombinantly expressed in insect and mamalian cells, displayed selective reduction of UDP-GlcNAc 2-epimerase activity by the loss of its tetrameric state, which is essential for full enzyme activity.
Regulation and pathophysiological implications of UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) as the key enzyme of sialic acid biosynthesis
TLDR
GNE knock-out in mice leads to embryonic lethality, emphasizing the crucial role of this key enzyme for sialic acid biosynthesis.
The analysis of N-glycans of cell membrane proteins from human hematopoietic cell lines reveals distinctions in their pattern
TLDR
The key approach of this study is the comparative analysis of five hematopoietic cell lines for their N-glycosylation pattern, which highlights the occurrence of N-acetyllactosamine extensions on tetraantennary structures in GNE-deficient cell lines.
Analysis of Cell Surface N-glycosylation of the Human Embryonic Kidney 293T Cell Line
TLDR
A high overall amount of sialylated N-glycans and the expression ofα2,3- and α2,8-specific, but not α 2,6- specific, sIALyltransferases were found.