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Autoinhibition is a recurring mode of protein kinase regulation and can be based on diverse molecular mechanisms. Here, we show by crystal structure analysis, nuclear magnetic resonance (NMR)-based nucleotide affinity studies and rational mutagenesis that nonphosphorylated mitogen-activated protein (MAP) kinases interacting kinase (Mnk) 1 is autoinhibited(More)
Red/ET recombination allows for extensive and base-precise manipulations of the Escherichia coli genome. Here we describe how this technology can be applied to improve the performance of the common expression host E. coli BL21(DE3): deletion of the DE3 sequence dispensable for T7-based gene expression enhances strain safety while recovery of the chromosomal(More)
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