Stefan Beyer

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The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including the anti-cancer metabolite epothilone. We report the complete genome sequence of the model Sorangium strain S. cellulosum So ce56, which produces several natural products and has morphological and physiological properties typical of the(More)
In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was found to have various advantageous(More)
Myxobacteria are gram-negative bacteria which are most noted for their ability to form fruiting bodies upon starvation. Within the last two decades, they increasingly gained attention as producers of natural products with biological activity. Here, recent and future biotechnological research on certain key myxobacteria and on their ability to produce(More)
During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed function derived from amino acid sequence(More)
Replicated systems that run over partitionable environments, can exhibit increased availability if isolated partitions are allowed to optimistically continue their execution independently. This availability gain is traded against consistency, since several replicas of the same objects could be updated separately. Once partitioning terminates, divergences in(More)
Two divergently oriented operons,strXU andstrVW, located within the gene cluster for 5′-hydroxystreptomycin (5′-OH-Sm) biosynthesis inStreptomyces glaucescens strain GLA.0 (ETH 22794), were analysed by DNA sequencing and transcription/regulation studies. Three genes,strU andstrVW, are conserved in a similar arrangement but in a different location within(More)
Natural products constitute important lead structures in drug discovery. In bacteria, they are often synthesized by large, modular multienzyme complexes. Detailed analysis of the biosynthetic machinery should enable its directed engineering and production of desirable analogs. The myxobacterium Sorangium cellulosum So ce90 produces the cytotoxic spiroketal(More)
Rolling circle amplification (RCA) is an elegant biochemical method by which long single-stranded DNA molecules with a repeating sequence motif can be readily synthesized. In RCA, small circular single-stranded oligonucleotides serve as templates for the polymerization of the complementary strand. A DNA polymerase with an efficient strand displacement(More)
Owing to the intimate linkage of sequence and structure in nucleic acids, DNA is an extremely attractive molecule for the development of molecular devices, in particular when a combination of information processing and chemomechanical tasks is desired. Many of the previously demonstrated devices are driven by hybridization between DNA 'effector' strands and(More)
We present a system architecture which facilitates enhanced availability of tightly coupled distributed systems by temporarily relaxing constraint consistency. Three different types of consistency are distinguished in tightly coupled distributed systems - replica consistency, concurrency consistency, and constraint consistency. Constraint consistency(More)