Stanisław Cebula

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An algorithm for bacterial identification using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is being developed. This mass spectral fingerprint comparison algorithm is fully automated and statistically based, providing objective analysis of samples to be identified. Based on extraction of reference fingerprint ions from test(More)
Bispeptide nucleic acids (bis-PNAs; PNA clamps), PNA oligomers, and DNA oligonucleotides were evaluated as affinity purification reagents for subfemtomolar 16S ribosomal DNA (rDNA) and rRNA targets in soil, sediment, and industrial air filter nucleic acid extracts. Under low-salt hybridization conditions (10 mM NaPO(4), 5 mM disodium EDTA, and 0.025% sodium(More)
The efficacy of PNA vs DNA oligomers for the recovery of femtomolar concentrations of 16S rDNA targets was determined with solution- and mixed-phase hybridization formats and limiting dilution quantitative PCR. Several results contradict existing perceptions of expected PNA behavior deduced from hybridization studies with oligonucleotide targets at high(More)
The aim of this paper has been to investigate the occurrence of health-promoting antioxidants, such as vitamins C and E as well as antioxidative enzymes, e.g. superoxide dismutase, catalase and peroxidase in different parts of the cabbage head (the outer one green leaves, the central leaf layer and the inner layer young, etiolated leaves). Vitamin C content(More)
The growth response and antioxidant capacity of Brassica oleracea var. capitata f. alba plants treated with 70ppb of ozone was examined. Four week old cabbage seedlings were fumigated with O3 for 3 days before being transplanted into the growing field. The effect of O3 treatment was determined directly after fumigation and over the course of field(More)
Abiotic stresses affect plant development in different ways depending on the phase of ontogeny. Exposure to moderate stress may induce cross-tolerance resulting in complex acclimation. The improvement of vegetable transplants able to withstand outdoor conditions is achieved by controlled stress applications. The aim of the experiment was to investigate the(More)
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