Photo-activation localization microscopy is a far-field superresolution imaging technique based on the localization of single molecules with subdiffraction limit precision. Known under acronyms such as PALM (photo-activated localization microscopy) or STORM (stochastic optical reconstruction microscopy), these techniques achieve superresolution by allowing… (More)
We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digital processing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object… (More)
We demonstrate an integrated holographic optical tweezers system with double-helix point spread function (DH-PSF) imaging for high precision three-dimensional multi-particle tracking. The tweezers system allows for the creation and control of multiple optical traps in three-dimensions, while the DH-PSF allows for high precision, 3D, multiple-particle… (More)
Appropriate PSF engineering in microscopy can be used to increase resolution , reduce aberrations  or to extend the depth-of-field (DOF) [3,4,5]. This study concentrates on PSF engineering in EDF microscopy. Most extended DOF techniques require image restoration by e.g. regularized filtering to achieve high lateral resolution and extended DOF… (More)
We present methods to improve the localization accuracy in wide-field 3D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3D localization, the estimation algorithms, and polarization sensitive detection.
The double-helix point spread function is much more sensitive to variation in depth (z). Superresolution images of DNA locations within bacteria 2D view: fluorescence (red) overlaid with white light (gray)