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Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
TLDR
Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet. Expand
5′‐Adenosinephosphosulphate reductase (CysH) protects Mycobacterium tuberculosis against free radicals during chronic infection phase in mice
TLDR
Results show that CysH is important for Mtb protection during the chronic infection phase, and that resistance to nitrosative and oxidative stress may be the mechanism of this protection. Expand
5′-Adenosinephosphosulfate Lies at a Metabolic Branch Point in Mycobacteria*
TLDR
Two conserved sequence motifs, CCXXRKXXPL and SXGCXXCT, may be used to predict the substrate specificity of these enzymes, and show that M. tuberculosis most likely produces PAPS for the sole use of this organism's sulfotransferases. Expand
Trehalose Is Required for Growth of Mycobacterium smegmatis*
TLDR
A dual role for trehalose as both a thermoprotectant and a precursor of critical cell wall metabolites is suggested in mycobacteria. Expand
Compartmentalization of Lipid Biosynthesis in Mycobacteria*
TLDR
Evidence is provided that enzymes involved in the biosynthesis of two major lipid classes, the phosphatidylinositol mannosides (PIMs) and aminophospholipids, are compartmentalized within the plasma membrane, and that polar PIM biosynthesis occurs in the plasma membranes rather than the cell wall component of the PM-CW. Expand
Aspartate 313 in the Streptomyces plicatusHexosaminidase Plays a Critical Role in Substrate-assisted Catalysis by Orienting the 2-Acetamido Group and Stabilizing the Transition State*
TLDR
Structural and kinetic data provide evidence that Asp-313 stabilizes the transition states flanking the oxazoline intermediate and also assists to correctly orient the 2-acetamido group for catalysis. Expand
Sulfotransferases and sulfatases in mycobacteria.
TLDR
A sequence-based analysis of newly discovered mycobacterial sulfotransferase genes, named stf1-stf10, is presented and Interestingly, two sulfOTransferases genes are highly similar to mammalian sulfotranferases, increasing the list of myc Cobacterial eukaryotic-like protein families. Expand
Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont.
TLDR
It is shown that GH92 mannosidases are alpha-mannosidases that act via a single displacement mechanism to utilize host N-glycans to exploit dietary polysaccharides and host glycans as nutrients. Expand
A semi-invariant Vα10+ T cell antigen receptor defines a population of natural killer T cells with distinct glycolipid antigen–recognition properties
TLDR
A population of α-GalCer-reactive NKT cells that expressed a canonical Vα10-Jα50 TCR α-chain showed a preference for α-glucosylceramide (α-GlcCer) and bacterial α- glucuronic acid–containing glycolipid antigens, which provides new insight into the structural basis and evolution of glycolIPid antigen recognition. Expand
Direct Evidence for ArOS Bond Cleavage upon Inactivation of Pseudomonas aeruginosa Arylsulfatase by Aryl Sulfamates
TLDR
Studying the inactivation of Pseudomonas aeruginosa arylsulfatase A by a range of aryl sulfamates found the stoichiometry of inactivation to be in the range 3–6, with the greatest values found for the most effective inactivators, providing valuable insight into the mechanism of sulfatase inactivation by sulfamate. Expand
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