Sotirios Kolivas

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Using Nycodenz, a novel density gradient medium, we isolated intact protein bodies from developing seeds of Lupinus angustifolius L. (cultivar Unicrop) and achieved excellent separation from the endoplasmic reticulum, mitochondria, and other organelles. The distribution of the storage protein conglutin-beta was taken as evidence that up to 96% of the(More)
The biosynthesis of conglutin δ has been studied in developing cotyledons of Lupinus angustifolius L. Precursors of conglutin δ formed the major sink for [35S]-cysteine incorporated by developing lupin cotyledons, and these precursors were rapidly sequestered into the endoplasmic reticulum. The sequence of a cDNA clone coding for one such precursor of(More)
Recombinant proteins containing amino acid sequences from open reading frame (ORF) 2 and ORF3 of a Chinese strain of hepatitis E virus (HEV) were constructed as fusions with glutathione S-transferase (GST). Stable fusion proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins were transferred to nitrocellulose(More)
The sequence of cDNA coding for a sulphur-rich storage protein from Lupinus angustifolius L., conglutin gamma, was determined. The coding region contained an N-terminal leader peptide of 28 amino acids which directly preceded subunits of M(r) 28,239 and 16,517. Extensive sequence homology between the protein encoded by conglutin gamma cDNA and basic 7S(More)
The sequence of cDNA coding for a sulphur-rich storage protein from Lupinus angustifolius L., conglutin γ, was determined. The coding region contained an N-terminal leader peptide of 28 amino acids which directly preceded subunits of M r 28 239 and 16 517. Extensive sequence homology between the protein encoded by conglutin γ cDNA and basic 7S globulin from(More)
In the absence of somatostatin antagonists, somatostatin antisera administered acutely or animals chronically immunized against somatostatin have been used to define the functions of somatostatin. However, the circulating immunoglobulins from immunized animals may contain substantial quantities of endogenous hormones. This has not been examined for(More)
Acid secretion first appears in the stomach during the later stages of fetal development. Gastric acid secretion is regulated by the stimulatory effects of gastrin, histamine, acetylcholine and the inhibitory actions of somatostatin on their respective receptors. A semi-quantitative reverse transcriptase-polymerase chain reaction method for the(More)
Gastric acid secretion is regulated by the stimulatory effects of gastrin, histamine and acetylcholine and the inhibitory actions of somatostatin on their respective receptors. We proposed that the expression of these receptors could be regulated at the transcription level by agonists and antagonists known to effect acid secretion. A quantitative(More)
BACKGROUND AND AIMS Gastric acid secretion is stimulated by the action of gastrin, histamine and acetylcholine on their respective receptors. To determine the regulation of synthesis of these receptors during different gastric secretory states a competitive RT-PCR method for quantitating the mRNA for these receptors was developed. METHODS Partial cDNA(More)
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