Sonja A. Wilhelm

Learn More
This report describes a real-time polymerase chain reaction assay with SYBR Green for detection of a broad range of porcine parvoviruses (PPV) and accurate virus quantification in porcine tissues. The assay targets the VP2 gene of PPV and the porcine genomic c-myc gene for normalization. The detection limit of the SYBR Green reaction was shown to be(More)
BACKGROUND Detection of parapoxviruses is important in various animals as well as in humans as zoonotic infections. Reliable detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, for both veterinarians and medical practitioners. To date, however, no real-time PCR assay for the detection of parapoxviruses has been(More)
Place attachment and recreation experience preferences (REP) have received increasing attention in natural resource management, with previous literature (Anderson & Fulton, 2008; Kyle, Mowen, & Tarrant, 2004) indicating that REP predicts place attachment development. This study expands current insight into the relationship between the two concepts.(More)
This report describes a real-time reverse transcription polymerase chain reaction (RT-PCR) assay with SYBR Green targeting the VP2 (ORF 3) of feline caliciviruses. All of the 44 feline calicivirus isolates tested were detected, whereas neither feline herpesvirus, feline panleukopenia virus, feline immunodeficiency virus and feline leukemia virus nor other(More)
  • 1