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A prokaryotic expression recombinant plasmid pET-PIB to express porin B (PIB) ofNeisseria gonorrhoeae in E. coli DE3 was constructed in order to provide a basis of research in detection, prophylactic and therapeutic vaccine against the pathogen infection. The gene encoding PIB was amplified by PCR fromNeisseria gonorrhoeae and cloned into prokaryotic(More)
In order to provide a rational research basis for clinical detection and genetic engineering vaccine, plasmid pET-28a (+) encoding both Porin gene PIA and PIB ofNeisseria gonorrhoeae was constructed and a fusion protein inE. coli DE3 expressed. The fragments of PIA and PIB gene ofNeisseria gonorrhoeae were amplified and cloned into prokaryotic expression(More)
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