Soichi Nakashiro

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BACKGROUND Preventing atherosclerotic plaque destabilization and rupture is the most reasonable therapeutic strategy for acute myocardial infarction. Therefore, we tested the hypotheses that (1) inflammatory monocytes play a causative role in plaque destabilization and rupture and (2) the nanoparticle-mediated delivery of pitavastatin into circulating(More)
OBJECTIVE Inflammatory monocytes/macrophages produce various proteinases, including matrix metalloproteinases, and degradation of the extracellular matrix by these activated proteinases weakens the mechanical strength of atherosclerotic plaques, which results in a rupture of the plaque. Peroxisome proliferator-activated receptor-γ induces a polarity shift(More)
Analysis of single-strand conformation polymorphism (SSCP) by capillary electrophoresis (CE) was developed. The conformational change of single-strand DNA is caused by a mutation in a DNA fragment. The change is detected as mobility shift in CE. The effects of acrylamide gel concentration, running temperature and fragment size amplified by the polymerase(More)
We describe the development of a sensitive high-performance liquid chromatographic (HPLC) method for polymerase chain reaction (PCR) products using bisbenzimide (Hoechst 33258 dye) based fluorimetric detection. The detection limit and specificity for double-strand DNA detection are improved in comparison with HPLC with UV absorbance detection. This HPLC,(More)
OBJECTIVES We sought to investigate whether treatment with ezetimibe in combination with statins improves coronary endothelial function in target vessels in coronary artery disease patients after coronary stenting. APPROACH AND RESULTS We conducted a multicenter, prospective, randomized, open-label, blinded-end point trial among 11 cardiovascular(More)
C oronary heart disease is the leading cause of death worldwide , and at least 7 million patients die of this disease each year (386 324 people in the United States alone). 1 Acute myo-cardial infarction (AMI) is the most severe type of coronary heart disease and the most frequent cause of heart failure, and it impairs the quality of life and inflates(More)
C oronary heart disease is the leading cause of death worldwide , and at least 7 million patients die of this disease each year (386 324 people in the United States alone). 1 Acute myo-cardial infarction (AMI) is the most severe type of coronary heart disease and the most frequent cause of heart failure, and it impairs the quality of life and inflates(More)
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