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The crystal structure of the tungsten-containing aldehyde ferredoxin oxidoreductase (AOR) from Pyrococcus furiosus, a hyperthermophilic archaeon (formerly archaebacterium) that grows optimally at 100 degrees C, has been determined at 2.3 angstrom resolution by means of multiple isomorphous replacement and multiple crystal form averaging. AOR consists of two(More)
There has been an increase in the amount of multilingual text on the Internet due to the proliferation of news sources and blogs. The Urdu language, in particular, has experienced explosive growth on the Web. Text mining for information discovery, which includes tasks such as identifying topics, relationships and events, and sentiment analysis, requires(More)
The goal of this work is to produce a classifier that can distinguish subjective sentences from objective sentences for the Urdu language. The amount of labeled data required for training automatic classifiers can be highly imbalanced especially in the multilingual paradigm as generating annotations is an expensive task. In this work, we propose a(More)
In this paper we propose a new approach to capture the inclination towards a certain election candidate from the contents of blogs and to explain why that inclination may be so. The method is based on the availability of "ground truth" speeches from the election candidates that are labeled and also on the collection of noisy blogs which are not labeled in(More)
The hyperthermophilic archaea Pyrococcus furiosus and Thermococcus litoralis contain the tungstoenzymes aldehyde ferredoxin oxidoreductase, a homodimer, and formaldehyde ferredoxin oxidoreductase, a homotetramer. herein we report the cloning and sequencing of the P. furiosus gene aor (605 residues; M(r), 66,630) and the T. litoralis gene for (621 residues;(More)
The hyperthermophilic archaeon Pyrococcus furiosus grows optimally at 100(deg)C by the fermentation of peptides and carbohydrates. From this organism, we have purified to homogeneity an intracellular protease, previously designated PfpI (P. furiosus protease I) (S. B. Halio, I. I. Blumentals, S. A. Short, B. M. Merrill, and R. M. Kelly, J. Bacteriol.(More)
In this paper we explore the possibility of using cross lingual projections that help to automatically induce role-semantic annotations in the PropBank paradigm for Urdu, a resource poor language. This technique provides annotation projections based on word alignments. It is relatively inexpensive and has the potential to reduce human effort involved in(More)
Three different types of tungsten-containing enzyme have been previously purified from Pyrococcus furiosus (optimum growth temperature, 100 degrees C): aldehyde ferredoxin oxidoreductase (AOR), formaldehyde ferredoxin oxidoreductase (FOR), and glyceraldehyde-3-phosphate oxidoreductase (GAPOR). In this study, the organism was grown in media containing added(More)
Automatic extraction of opinion holders and targets (together referred to as opinion entities) is an important subtask of sentiment analysis. In this work, we attempt to accurately extract opinion entities from Urdu newswire. Due to the lack of resources required for training role labelers and dependency parsers (as in English) for Urdu, a more robust(More)