Smita S. Patel

Learn More
Bacteriophage T7 gp4 serves as a model protein for replicative helicases that couples deoxythymidine triphosphate (dTTP) hydrolysis to directional movement and DNA strand separation. We employed single-molecule fluorescence resonance energy transfer methods to resolve steps during DNA unwinding by T7 helicase. We confirm that the unwinding rate of T7(More)
ALOHA, samples were amplified by polymerase chain reaction (PCR) with a nested set of degenerate nifH primers 19,20. The resulting PCR products were cloned and sequenced to characterize the nifH sequence diversity. Detailed descriptions of the methods used for the PCR amplification, cloning and sequencing are found in ref. 19. A simple, high precision, high(More)
  • 1