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Enamel Matrix Protein Interactions

This work believes that each of these enamel matrix proteins must interact with other organic components of ameloblasts and the enamel Matrix, likely protein partners would include integral membrane proteins and additional secreted proteins.
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TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly

TFIP11 recruits mDEAH9 suggesting that these two proteins have similar biological activities to their yeast counterparts, based on amino-acid sequence similarities across species.
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Identification of a novel nuclear localization signal and speckle-targeting sequence of tuftelin-interacting protein 11, a splicing factor involved in spliceosome disassembly.

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TFIP11, CCNL1 and EWSR1 Protein-protein Interactions, and Their Nuclear Localization

Using fluorescently-tagged proteins and confocal microscopy, it is shown that TFIP11, CCNL1 and EWSR1 frequently co-localize to speckled nuclear domains, which would suggest that all three proteins participate in a common cellular activity related to RNA splicing events.
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Using the yeast two-hybrid assay to discover protein partners for the leucine-rich amelogenin peptide and for tuftelin-interacting protein 11.

The sensitive yeast two-hybrid assay was used to identify proteins that interact directly with amelogenin, ameloblastin, enamelin, the leucine-rich amelgenin peptide (LRAP) and TFIP11.
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An amelogenin minigene to study alternative splicing.

This work has successfully cloned an amelogenin minigene, and as a result it was shown to be functionally active by transfection into multiple cell lines and to describe and discuss novel amelgenin alternatively spliced transcripts.
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Detection of mycoplasma contamination in cell cultures

It is recommended that combination of at least 2 methods should be performed to detect mycoplasma contamination, and there are many detection methods available such as microbiological culture, DNA staining, PCR, and biochemical assays.

Effects of tannin-fluoride and milk-fluoride mixture on human enamel erosion from inappropriately chlorinated pool water.

It is demonstrated that treatment with fluoridated milk with or without tannin-fluoride has protective effects against enamel erosion caused by low-pH swimming pool water.
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Expression of translationally controlled tumor protein in heat-stressed human dental pulp cells.

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Long-Term Effect of Modified Glass Ionomer Cement with Mimicked Biological Property of Recombinant Translationally Controlled Protein

This study modified glass ionomer cement (GIC) by adding mimicked biological molecules to reduce cell death. GIC was modified to BIOGIC by adding chitosan and bovine serum albumin for enhancing…
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