Siriporn Pongsomboon

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A large-scale expressed sequence tag (EST) sequencing project was undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. Initially, 15 cDNA libraries were constructed from different tissues (eyestalk, hepatopancrease, haematopoietic tissue, haemocyte, lymphoid organ, and ovary) of shrimp, reared under normal or stress(More)
Random amplified polymorphic DNA (RAPD) analysis was used to amplify the genome of black tiger prawns (Penaeus monodon) to detect DNA markers and assess the utility of the RAPD method for investigating genetic variation in wild P. monodon in Thailand. A total of 200 ten-base primers were screened, and 84 primers yielded amplification products. Six positive(More)
The lymphoid organ of penaeid shrimps is proposed to play an important role in the innate immune system. To investigate the potential immune function of the lymphoid organ, we analyzed the expressed genes from the lymphoid organ of normal and Vibrio harveyi-infected Penaeus monodon using an expressed sequence tag (EST) approach. Sequence analysis of the EST(More)
Suppression subtractive hybridization (SSH) was employed to identify yellow head virus (YHV)-responsive genes from the hemocytes of the black tiger shrimp, Penaeus monodon. Two SSH cDNA libraries were constructed to identify viral responsive genes in the early (24I) and late (48/72I) phases of YHV infection. From 240 randomly selected clones from each(More)
In this study, microsatellite markers were developed for the genetic linkage mapping and breeding program of the black tiger shrimp Penaeus monodon. A total of 997 unique microsatellite-containing expressed sequence tags (ESTs) were identified from 10 100 EST sequences in the P. monodon EST database. AT-rich microsatellite types were predominant in the EST(More)
A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One(More)
A cDNA microarray comprised of 9990 different ESTs obtained from the Penaeus monodon EST project (http://pmonodon.biotec.or.th) was employed to identify viral (white spot and yellow head viruses) and bacterial (Vibrio harveyi) responsive genes in the hemocytes of P. monodon at 6, 24 and 48 h post-injection (hpi). The number of differentially expressed genes(More)
Two isoforms of 14-3-3 protein, namely 14-3-3A and 14-3-3B, from the shrimp Penaeus monodon were investigated for their potential role in adaptation to salinity stress. Transcripts of 14-3-3A were found in various shrimp tissues whilst expression of 14-3-3B transcripts was more specific being observed in the osmoregulatory tissues, that is in the gills and(More)
In order to characterize triand tetranucleotide microsatellites in the black tiger prawn, Penaeus monodon, a genomic library of P. monodon was screened with the repeats (GAA)n, (GATA)n, (GGAT)n, (GGAA)n and (CACC)n. In the 79 positive clones isolated, the repeats (GATA)n was found at the highest frequency, followed by the (GAA)n while the other sequence(More)
Carbonic anhydrase (CA) was identified by differential display PCR analysis as one of the differentially expressed genes in the gills of low salinity stressed (transferred from 25 to 3 ppt) Penaeusmonodon. To further characterize the role of CA in the regulation of salinity stress, the cDNA sequence of P.monodon carbonic anhydrase (PmCA) was attained by(More)