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Influenza viruses exploit host cell machinery to replicate, resulting in epidemics of respiratory illness. In turn, the host expresses antiviral restriction factors to defend against infection. To find host cell modifiers of influenza A H1N1 viral infection, we used a functional genomic screen and identified over 120 influenza A virus-dependency factors(More)
To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West(More)
The 2009 H1N1 influenza pandemic showed the speed with which a novel respiratory virus can spread and the ability of a generally mild infection to induce severe morbidity and mortality in a subset of the population. Recent in vitro studies show that the interferon-inducible transmembrane (IFITM) protein family members potently restrict the replication of(More)
  • Richard G Guy, Thomas W Page, S John, Gerald J Heidemann, Popek
  • 1990
Previous distributed le systems have relied on either convention or obtaining dynamic global agreement to provide network transparent le naming. This paper argues that neither approach can succeed as systems scale to the kind of size that is anticipated in the current decade. We propose instead a novel name mapping scheme which relies on a fragmented,(More)
RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and false negatives. To ameliorate these issues, we combined information from the existing HDF screens together with new screens performed with multiple orthologous RNAi reagents (MORR). In(More)
Macrophages play a critical role in the innate immune response to pathogen infection, but few tools exist for systematic dissection of these responses using modern genome-wide perturbation methods. To develop an assay platform for high-throughput analysis of macrophage activation by pathogenic stimuli, we generated reporter systems in human and mouse(More)
  • Gerald J Popek, Richard G Guy, Thomas W Page, S John, Heidemann
  • 1990
Ficus is a replicated general ling environment for Unix intended to scale to very large (nationwide) networks. The system employs an optimistic \one copy availability" model in which connicting updates to the le system's directory information are automatically reconciled, while connicting le updates are reliably detected and reported. The system(More)
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