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Cinnamoyl CoA:NADP oxidoreductase (CCR, EC 1.2.1.44) catalyzes the conversion of cinnamoyl CoA esters to their corresponding cinnamaldehydes, i.e. the first specific step in the synthesis of the lignin monomers. The cloning of a cDNA encoding CCR in Eucalyptus gunnii (EUCCR) is reported here. The identity of the EUCCR cDNA was demonstrated by comparison(More)
We have proposed replicator neural networks (RNNs) for outlier detection [8]. Here we compare RNN for out-lier detection with three other methods using both publicly available statistical datasets (generally small) and data mining datasets (generally much larger and generally real data). The smaller datasets provide insights into the relative strengths and(More)
Flax (Linum usitatissimum) is an ancient crop that is widely cultivated as a source of fiber, oil and medicinally relevant compounds. To accelerate crop improvement, we performed whole-genome shotgun sequencing of the nuclear genome of flax. Seven paired-end libraries ranging in size from 300 bp to 10 kb were sequenced using an Illumina genome analyzer. A(More)
Lignin and lignans share monolignols as common precursors and are both potentially involved in plant defence against pathogens. In this study, we investigated the effects of fungal elicitors on lignin and lignan metabolism in flax (Linum usitatissimum) cell suspensions. Cell suspension cultures of flax were treated with elicitor preparations made from(More)
BACKGROUND Quantitative real-time PCR (qRT-PCR) is currently the most accurate method for detecting differential gene expression. Such an approach depends on the identification of uniformly expressed 'housekeeping genes' (HKGs). Extensive transcriptomic data mining and experimental validation in different model plants have shown that the reliability of(More)
We consider the problem of finding outliers in large multi-variate databases. Outlier detection can be applied during the data cleansing process of data mining to identify problems with the data itself, and to fraud detection where groups of outliers are often of particular interest. We use replicator neural networks (RNNs) to provide a measure of the(More)
The nature of the enzyme(s) involved in the dehydrogenative polymerization of lignin monomers is still a matter of debate. Potential candidates include laccases which have recently received attention due to their capacity to oxidize lignin monomers and close spatial and temporal correlation with lignin deposition. We have characterized two H2O2-independent(More)
BACKGROUND Flax (Linum usitatissimum L.) has been cultivated for around 9,000 years and is therefore one of the oldest cultivated species. Today, flax is still grown for its oil (oil-flax or linseed cultivars) and its cellulose-rich fibres (fibre-flax cultivars) used for high-value linen garments and composite materials. Despite the wide industrial use of(More)
MicroRNAs (miRNAs) are small non-protein coding regulatory RNAs released after the cleavage of a primary transcript. A computational homology search of expressed sequence tags (ESTs) available in public databases allowed the identification of 20 conserved miRNAs belonging to 13 different families in flax (Linum usitatissimum). Most of the miRNAs were 21(More)