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Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins.
Several binding scaffolds that are not based on immunoglobulins have been designed as alternatives to traditional monoclonal antibodies. Many of them have been developed to bind to folded proteins,Expand
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Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry
ABSTRACT Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dynamic execution of biological processes. While antibodies represent a well-establishedExpand
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Designed Armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity.
Designed Armadillo repeat proteins (ArmRPs) are a novel class of binding proteins intended for general modular peptide binding and have very favorable expression and stability properties. Using aExpand
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Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity
Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, immobilization or manipulation ofExpand
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Structure and Energetic Contributions of a Designed Modular Peptide-Binding Protein with Picomolar Affinity.
Natural armadillo repeat proteins (nArmRP) like importin-α or β-catenin bind their target peptides such that each repeat interacts with a dipeptide unit within the stretched target peptide. However,Expand
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Knowing You, Seeing Me: Investigating User Preferences in Drone-Human Acknowledgement
TLDR
We present three studies on drone gestures to acknowledge human presence and clarify suitable acknowledging distances. Expand
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G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins
We introduce designed ankyrin repeat binding proteins (DARPins) as a novel class of highly specific and structure-selective DNA-binding proteins, which can be functionally expressed within all cells.Expand
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Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects
Designed armadillo-repeat proteins are promising scaffolds for modular peptide-recognition systems. The crystal structures of His-YIIIM4AII, His-YIIIM5AII and YIIIM5AII highlight structuralExpand
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Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition.
Armadillo repeat proteins (ArmRPs) recognize their target peptide in extended conformation and bind, in a first approximation, two residues per repeat. Thus, they may form the basis for building aExpand
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Structures of designed armadillo repeat proteins binding to peptides fused to globular domains
Designed armadillo repeat proteins (dArmRP) are α‐helical solenoid repeat proteins with an extended peptide binding groove that were engineered to develop a generic modular technology for peptideExpand
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