Simon F. Tolic-Norrelykke

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We explain and demonstrate a new method of force-and position-calibration for optical tweez-ers with back-focal-plane photo detection. The method combines power spectral measurements of thermal motion and the response to a sinusoidal motion of a translation stage. It consequently does not use the drag coefficient of the trapped object as an input. Thus,(More)
The first half of this chapter describes the development in mathematical models of Brownian motion after Einstein's seminal papers and current applications to optical tweezers. This instrument of choice among single-molecule biophysicists is also an instrument of precision that requires an understanding of Brownian motion beyond Einstein's. This is(More)
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