Simina Ticau

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Loading of the ring-shaped Mcm2-7 replicative helicase around DNA licenses eukaryotic origins of replication. During loading, Cdc6, Cdt1, and the origin-recognition complex (ORC) assemble two heterohexameric Mcm2-7 complexes into a head-to-head double hexamer that facilitates bidirectional replication initiation. Using multi-wavelength single-molecule(More)
Viral hijacking of cellular processes relies on the ability to mimic the structure or function of cellular proteins. Many viruses encode ubiquitin ligases to facilitate infection, although the mechanisms by which they select their substrates are often unknown. The Herpes Simplex Virus type-1-encoded E3 ubiquitin ligase, ICP0, promotes infection through(More)
Eukaryotic replication initiation is highly regulated and dynamic. It begins with the origin recognition complex (ORC) binding DNA sites called origins of replication. ORC, together with Cdc6 and Cdt1, mediate pre-replicative complex (pre-RC) assembly by loading a double hexamer of Mcm2-7: the core of the replicative helicase. Here, we use single-molecule(More)
The opening and closing of two ring-shaped Mcm2-7 DNA helicases is necessary to license eukaryotic origins of replication, although the mechanisms controlling these events are unclear. The origin-recognition complex (ORC), Cdc6 and Cdt1 facilitate this process by establishing a topological link between each Mcm2-7 hexamer and origin DNA. Using(More)
The authors have carried out a comparative analysis of the sensitivity of the microscopic examination as compared with that of the bacteriologic cultures in the evidencing of the Koch bacilli in biologic products obtained from new cases of tuberculosis, before the start of any kind of treatment with tuberculostatics. The authors attempted to establish the(More)
A series of mechanical factors, such as postural changes and every day movements, favour mobilization of bronchoalveolar secretions and, hence, offer greater chanses of detecting the tuberculosis bacillus. It is recommended to collect, in out-patient units, two sputum samples, one in the morning and the second several hours later, after beginning the day's(More)