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This study describes a comparison of the polyhydroxyalkanoate (PHA) synthases PhaC1 and PhaC2 of Pseudomonas mendocina. The P. mendocina pha gene locus, encoding two PHA synthase genes [phaC1Pm and phaC2Pm flanking a PHA depolymerase gene (phaZ)], was cloned, and the nucleotide sequences of phaC1Pm (1,677 bp), phaZ (1,034 bp), and phaC2Pm (1,680 bp) were(More)
Here we describe a novel microarray platform that integrates all functions needed to perform any array-based experiment in a compact instrument on the researcher's laboratory benchtop. Oligonucle otide probes are synthesized in situ via a light- activated process within the channels of a three-dimensional microfluidic reaction carrier. Arrays can be(More)
A recombinant strain of Escherichia coli was used to produce poly(4-hydroxybutyric acid), P(4HB), homopolyester by fed-batch culture in M9 mineral salts medium containing glucose and 4-hydroxybutyric acid as carbon sources. The final cell dry weight, P(4HB) concentration and P(4HB) content were 12.6 g/l, 4.4 g/l, and 36% of cell dry weight, respectively, in(More)
1. Solitary bees are central place foragers returning to their nests several times a day with pollen and nectar to provision their brood cells. They are especially susceptible to landscape changes that lead to an increased spatial separation of suitable nesting sites and flower rich host plant stands. While knowledge of bee foraging ranges is currently(More)
Animal movement behaviour is intensively investigated with capture-mark-recapture studies. For the analysis of such experiments, the influence of marking technique, handling and translocation of marked animals on movement pattern is of crucial importance since it may mask or overrule the effects of the main research question. Here we present a(More)
One goal of conservation biology is the assessment of effects of land use change on species distribution. One approach for identifying the factors, which determine habitat suitability for a species are statistical habitat distribution models. These models are quantitative and can be used for predictions in management scenarios. However, they often have one(More)
Intercontinental trade has led to multiple introductions of invasive pest species at a global scale. Molecular analyses of the structure of populations support the understanding of ecological strategies and evolutionary patterns that promote successful biological invasions. The oriental fruit moth, Grapholita (= Cydia) molesta, is a cosmopolitan and(More)
To investigate whether landscape structures act as insurmountable barriers for foraging bees, we conducted mark-recapture studies with two pollen-specialist solitary species. Foraging options of the bees were confined to host plant stands across different landscape structures. Differences in altitude of more than 130 m were overcome and forests covering a(More)
Field Station Fabrikschleichach, Glashuettenstrasse 5, 96181 Rauhenebrach, Germany UFZ – Centre for Environmental Research Leipzig-Halle, Dept. of Conservation Biology, Permoserstr. 15, 04318 Leipzig, Germany IBE, Landscape Ecology Group, Carl von Ossietzky University of Oldenburg, PO Box 2503, 26111 Oldenburg, Germany UFZ – Centre for Environmental(More)
During cultivation under storage conditions with BG11 medium containing acetate as a carbon source, Synechocystis sp. PCC6803 accumulated poly(3-hydroxybutyrate) up to 10% (w/w) of the cell dry weight. Our analysis of the complete Synechocystis sp. PCC6803 genome sequence, which had recently become available, revealed that not only the open reading frame(More)