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We systematically generated large-scale data sets to improve genome annotation for the nematode Caenorhabditis elegans, a key model organism. These data sets include transcriptome profiling across a developmental time course, genome-wide identification of transcription factor-binding sites, and maps of chromatin organization. From this, we created more(More)
Analysis of genetic interactions has been extensively exploited to study gene functions and to dissect pathway structures. One such genetic interaction is synthetic lethality, in which the combination of two non-lethal mutations leads to loss of organism viability. We have developed a dSLAM (heterozygote diploid-based synthetic lethality analysis with(More)
Peroxisome proliferators are known to modulate the activity of xenobiotic-metabolising enzymes, including glutathione S-transferase (GST) and cytochrome P-450 (CYP). In this study the effect of peroxisome proliferators silvex and di(2-ethylhexyl)phthalate (DEHP) on the formation of (+)-anti-benzo(a)pyrene -7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts(More)
trans-7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(+)-anti-BPDE] is rapidly inactivated in aqueous solvents due to hydrolysis to tetraols. No significant effect on the rate of hydrolysis is observed in the presence of glutathione (GSH)-depleted cytosol. However, when the cytosolic fraction is replaced by a mixture of glutathione(More)
Glutathione S-transferase (GST) isoenzymes of rat liver cytosol were purified from a S-hexylglutathione Sepharose affinity column without using S-hexyl-glutathione for elution. The method involves an initial elution of GSTs 3-4 and 4-4 from the affinity matrix with 10 mM glutathione in 50 mM Tris-HCl (pH 7.0), followed by the elution of GSTs 1-1, 1-2, 2-2(More)
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