Sidney Weinhouse

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In order to identify intermediates of CCl4 metabolism, whole, suitably fortified rat liver homogenates were incubated with 14CCl4 in the presence and absence of "pools" of unlabeled suspected intermediates. In the presence of NADH or NADPH, incorporation of radioactivity was rapid and substantial in CO2, lipid, protein, and the acid-soluble fraction. It was(More)
We propose a general model for neoplastic development which postulates that the loss of methyl groups from 5-methylcytosines (5-mC) involved in the control of gene expression may initiate neoplastic transformation and give rise to the aberrant phenotype of the transformed cell. Interference with normal patterns of methylation can be envisioned to occur by a(More)
Inorganic pyrophosphatase (EC has been purified to electrophoretic homogeneity from the soluble portion of the cytoplasm of rat Hepatoma 3924A and rat liver. It has a specific activity of 600 to 700 mumol inorganic orthophosphate liberated per min per mg protein at 25 degrees, a value in the same range as the highly purified enzymes from yeast and(More)
SUMMARY In a continuation of studies on glycolytic and respiratory interrelationships in a series of rat hepatomas ranging widely in growth rate and degree of differentiation, whole, fortified homogenates of these tissues were incubated at 28°Cin the presence of frue tose-1,6-diphosphate (FDP), 2-deoxyglucose (2-DG), and exogenous hexokinase; respiration,(More)
cal composition of DNA or in one of the â€oeregulator†• substances remains uncertain (3, 6). If there is a specific site of cancer initiation in the genome, the neoplastic transformation should be associated with specific bio chemical aberrations. If such exist, they have not yet been uncovered. Perhaps the closest approach to a bio chemical phenotype of(More)
SUMMARY Pyruvate kinase (PK) (EC isozymes were assayed in normal rat liver and a series of transplantable rat hepatomas ranging widely in growth rate and degree of differentiation, with the use of gradient elution by chloride ion from columns of DEAE-cellulose. In agreement with other studies, three noninterconvertible forms were found in rat(More)
found that N-acetyl-L-glutamate-dependent CPS l@ EEC (NH4)], one of the 1st steps in the pathway of urea synthesis, was retained in varying degrees in well-and highly differentiated tumors (14). There was also a marked lowering of CPS I activity in the livers of rats bearing large, slow-growing tumors with high CPS I activity (21, 28A, 44, 47C, and(More)
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