Lipidomics reveals a remarkable diversity of lipids in human plasma1[S]
The focus of the present study was to define the human plasma lipidome and to establish novel analytical methodologies to quantify the large spectrum of plasma lipids and to quantitatively assessed the levels of over 500 distinct molecular species distributed among the main lipid categories.
Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe–2S] clusters
It is demonstrated that chloroplast CGFS Grxs have the potential to function as scaffold proteins for the assembly of [2Fe–2S] clusters that can be transferred intact to physiologically relevant acceptor proteins.
Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe–2S] cluster in poplar glutaredoxin C1
Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein indicate that theholoprotein contains a subunit-bridging [2Fe–2S] cluster that is ligated by the catalytic cysteine of two glutaredoxins and the cysteines of three glutathiones.
A Proposed Role for the Azotobacter vinelandii NfuA Protein as an Intermediate Iron-Sulfur Cluster Carrier*
- Sibali Bandyopadhyay, S. Naik, P. D. Dos Santos
- Biology, ChemistryJournal of Biological Chemistry
- 16 May 2008
Spectroscopic and analytical studies indicate that one [4Fe-4S] cluster can be assembled in vitro within a dimeric form of NfuA, which is competent for rapid in vitro activation of apo-aconitase.
Iron-sulfur cluster biosynthesis.
- Sibali Bandyopadhyay, K. Chandramouli, Michael K. Johnson
- Chemistry, BiologyBiochemical Society Transactions
- 1 December 2008
The present review highlights recent developments in understanding the mechanism of Fe-S cluster assembly and transfer involving the ubiquitous U-type scaffold proteins and the potential roles of accessory proteins such as Nfu proteins and monothiol glutaredoxins in the assembly, storage or transfer ofFe-S clusters.
Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation
It is shown that multiple hepatitis C virus genotypes are exquisitely sensitive to oxidative membrane damage, a property distinguishing them from other pathogenic RNA viruses, and that the typical, wild-type HCV replicase is uniquely regulated by lipid peroxidation.
Characterization and Functional Implications
Saccharomyces cerevisiae mitochondrial glutaredoxin 5 (Grx5) is the archetypical member of a ubiquitous class of monothiol glutaredoxins with a strictly conserved CGFS active-site sequence that has…
Ceramide sphingolipid signaling mediates Tumor Necrosis Factor (TNF)-dependent toxicity via caspase signaling in dopaminergic neurons
- Terina N. Martinez, X. Chen, Sibali Bandyopadhyay, A. Merrill, M. Tansey
- Biology, ChemistryMolecular Neurodegeneration
- 13 September 2012
It is concluded that TNF/TNFR1-dependent activation of SMases generates ceramide and sphingolipid species that promote degeneration and caspase-dependent cell death of DA neurons.
Acid Ceramidase (ASAH1) Represses Steroidogenic Factor 1-Dependent Gene Transcription in H295R Human Adrenocortical Cells by Binding to the Receptor
- N. Lucki, Donghui Li, Sibali Bandyopadhyay, E. Wang, A. Merrill, M. Sewer
- BiologyMolecular and Cellular Biology
- 27 July 2012
Results demonstrate that ASAH1 is a novel coregulatory protein that represses SF-1 function by directly binding to the receptor onSF-1 target gene promoters and identify a key role for nuclear lipid metabolism in regulating gene transcription.