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The use of highly specific and highly sensitive immunofluorescent probes is a promising approach for biomedical imaging in living tissue. We focus on immunofluorescence with quantum dot bioconjugates for hepatoma detection in vivo. We synthesized specific immunofluorescent probes by linking quantum dots to AFP (alpha-fetoprotein) antibody for specific(More)
A new class of fluorescent probe produced by conjugating semiconductor quantum dots (QDs) with protein molecule is proposed as an alternative to conventional organic labels. However the fluorescence characteristics of the QD bioconjugates are not clear while they are excitied with one- or two-photon laser pulse. We synthesized specific immunofluorescent(More)
They studied how the complex responded to the primary constituents of blood serum: DNA, RNA, proteins and glucose molecules. We found that the sensor was extremely selective towards DNA even in the presence of RNA and proteins. DNA could be detected fluorescently with parts-per-million accuracy. It turns out that only DNA has the ionic strength capable of(More)
To investigate the influence of surface trapping and dark states on CdSe and CdSe/ZnS quantum dots (QDs), we studied the absorption, fluorescence intensity and lifetime by using one-and two-photon excitation, respectively. Experimental results show that both one- and two-photon fluorescence emission efficiencies of the QDs enhance greatly and the lifetime(More)
We demonstrate highly efficient avalanche multiphoton luminescence (MPL) from ordered-arrayed gold nanowires (NWs) with low time-average excitation intensity, Iexc (5.0-9.1 kW/cm2). The intensity of avalanche MPL, IMPL, is about 10(4) times larger than that of three-photon luminescence, the slope partial differential log IMPL/ partial differential log Iexc(More)
We report on controlled synthesis of uniform LnVO4 (Ln = Ce and Nd) nanocrystals (NCs) with square-plate and H-shaped morphologies in nanosized reverse microemulsion reactors, via a facile solvo/hydrothermal strategy. The NCs were thoroughly characterized by X-ray diffraction, transmission electron microscopy (TEM), atomic force microscopy (AFM),(More)
A fluorescence resonance energy transfer (FRET) model using two-photon excitable small organic molecule DMAHAS as energy donor has been constructed and tried in an assay for avidin. In the FRET model, biotin was conjugated to the FRET donor, and avidin was labeled with a dark quencher DABS-Cl. Binding of DABS-Cl labeled avidin to biotinylated DMAHAS(More)
Spatial self-phase modulation (SSPM) experiments on two-dimensional (2D) black phosphorus (BP) nanoflake suspensions are performed with focused femtosecond pulsed lasers at 350-1160 nm. In the broadband region, the slope of the SSPM ring number versus laser intensity varies from 0.99 to 0.34, which is larger than 0.25 in MoS2. We deduce the portion of the(More)
We observe dynamic self-diffraction in MoS(2) supernatant solutions with laser for the first time, and conduct dynamic data simulation and analysis. Observation results indicate that self-diffraction can be divided in three stages: in the first stage, laser changes from Gauss beam to symmetric diffraction rings because of the force from laser. In the second(More)
Two strategies have been explored for organic functionalizations of ZnO nanotetrapods via anchoring groups of carboxylate and phosphonate. With these methods, oleyl chains were assembled on the surfaces of the ZnO nanotetrapods, significantly enhancing their solubility in nonpolar solvents, such as chloroform and toluene. The surface functionalization(More)