Shuxun Chen

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Sorting (or isolation) and manipulation of rare cells with high recovery rate and purity are of critical importance to a wide range of physiological applications. In the current paper, we report on a generic single cell manipulation tool that integrates optical tweezers and microfluidic chip technologies for handling small cell population sorting with high(More)
Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at(More)
Cell fusion is a fast and highly efficient technique for cells to acquire new properties. The fusion of somatic cells with stem cells can reprogram somatic cells to a pluripotent state. Our research on the fusion of stem cells and cancer cells demonstrates that the fused cells can exhibit stemness and cancer cell-like characteristics. Thus, tumor-initiating(More)
This paper presents a robot-assisted methodology that integrates optical tweezer and microfluidic chip technologies to realize automatic cell sorting from small sample population. The microfluidic chip used for cell sorter is designed and fabricated, and the flow environment within the microfluidic channel is investigated with simulation. Two image(More)
Isolation and deposition of specific live cells with the high spatio-temporal resolution from the heterogeneous mixtures are of critical importance to a wide range of biomedical applications. In this paper, we report a robot-assisted cell manipulation tool with optical tweezers based on a uniquely designed microwell array. The whole automatic manipulation(More)
Engineering induced cell fusion is becoming a promising tool in novel therapeutic studies for treating various diseases. The majority of current in vitro cell fusion methods are based on random cell pairing with loose contact, which also needs large amounts of cells. In this paper, we present a robotically controlled laser-induced cell fusion approach based(More)
Cell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value. Herein, we developed an automated micropipette-based quantitative microinjection technology that can deliver precise(More)
As rapid development of precision medicine, in vivo manipulation of micro/nano-scaled particles have attracted increasing attention in recent years. To accommodate complex in-vivo environment, robot-aided automated manipulation technology is highly demanded in trapping and controlling micro/nano-particles stably and effectively. This paper presents an(More)
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) provide an unlimited source of donor cells for potential cardiac regenerative therapies. However, hPSC-CMs are immature. For instance, hPSC-CMs are only 1/10 of the physical size of their adult counterparts; the majority are mono- rather than bi- or multi-nucleated, which is an evolutionary(More)
Single cell surgery has been gaining increasing attention due to its extensive applications in physiological and pathological of cell research. Orientation control of biological cell is a basic and vital technique required in cell surgery procedures. By adjusting the orientation of the cell appropriately, the components and sites of interest can be captured(More)