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Metabolic engineering of Escherichia coli for the biosynthesis of various phenylpropanoid derivatives.
Design and application of an in vivo reporter assay for phenylalanine ammonia-lyase
- Siyuan Wang, Shuwei Zhang, T. Zhou, Jia Zeng, J. Zhan
- BiologyApplied Microbiology and Biotechnology
- 2 August 2013
A novel and efficient visible reporter assay for screening of PAL efficiency in Escherichia coli was established based on a plant type III polyketide biosynthetic pathway and three PALs (PAL1, PAL3, and PAL4) were screened, among which PAL1 showed the best performance in E. coli.
Thioesterase domains of fungal nonreducing polyketide synthases act as decision gates during combinatorial biosynthesis.
- Yuquan Xu, T. Zhou, Shuwei Zhang, L. Xuan, J. Zhan, I. Molnár
- BiologyJournal of the American Chemical Society
- 24 July 2013
By exploiting chimeric iPKS enzymes to offer substrates with controlled structural variety to two orthologous O-C bond-forming TE domains in situ, it is shown that these enzymes act as nonequivalent decision gates, determining context-dependent release mechanisms and overall product flux.
Ganoderic acid C1 isolated from the anti-asthma formula, ASHMI™ suppresses TNF-α production by mouse macrophages and peripheral blood mononuclear cells from asthma patients.
Berberine and limonin suppress IgE production by human B cells and peripheral blood mononuclear cells from food-allergic patients.
Characterization of a methyltransferase involved in herboxidiene biosynthesis.
Characterization of Three Tailoring Enzymes in Dutomycin Biosynthesis and Generation of a Potent Antibacterial Analogue.
Dutomycin and SW91 showed strong antibacterial activity against Staphylococcus a Aureus and methicillin-resistant S. aureus, whereas POK-MD1 and SW75 had no obvious inhibition, which revealed the essential role of the C-4″ triketide chain in antib bacterial activity.
Three enzymes involved in the N-methylation and incorporation of the pradimicin sugar moieties.
Diversity-oriented combinatorial biosynthesis of benzenediol lactone scaffolds by subunit shuffling of fungal polyketide synthases
- Yuquan Xu, T. Zhou, I. Molnár
- Biology, ChemistryProceedings of the National Academy of Sciences
- 21 July 2014
Fungal benzenediol lactone (BDL) polyketides are important pharmacophores with wide-ranging bioactivities, including heat shock response and immune system modulatory effects, and their biosynthesis on a pair of sequentially acting iterative polyketide synthases (iPKSs) offers a test case for the modularization of secondary metabolic pathways into “build–couple–pair” combinatorial synthetic schemes.
Decoding and reprogramming fungal iterative nonribosomal peptide synthetases
The total biosynthesis of beauvericin in vitro is reconstructed by reacting C2 and C3 with two SNAC-linked precursors and a domain swapping approach to reprogramming these enzymes for peptides with altered lengths is presented.