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Pseudoalteromonas flavipulchra JG1 produces a protein PfaP and a range of small-molecule compounds with inhibitory activities against Vibrio anguillarum. The PfaP protein was purified from the extracellular products of JG1 by electroelution, and antibacterial activity was observed by an in-gel antibacterial assay. The complete amino acid sequence (694 aa)… (More)
Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.
Based on the technology of near-infrared spectroscopy, a novel near-infrared moisture content determination instrument has been developed with three laser diodes of different wavelengths as light source, with one InGaAs detector as determination device, and with integrating sphere as sample holding device. Besides, LabVIEW virtual instrument program was… (More)