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Multiple Mechanisms Are Involved in Repression of Filamentous Phage SW1 Transcription by the DNA-Binding Protein FpsR.
TLDR
Data support a model in which FpsR actively regulates phage production by interacting with the corresponding operators, thus playing a crucial role in the SW1 genetic switch.
Phylogenomic analysis reveals a two-stage process of the evolutionary transition of Shewanella from the upper ocean to the hadal zone.
TLDR
A two-stage process of the evolutionary transition of Shewanella from the upper ocean to the hadal zone is revealed by comparative genomics and gene gain/loss analysis and the transcriptomic analysis demonstrated that recently obtained genes are strictly repressed and may thus play a minor role in the response to environmental changes.
Validation of reference genes for reverse transcription real‐time quantitative PCR analysis in the deep‐sea bacterium Shewanella psychrophila WP2
TLDR
The rho gene is conserved in Shewanella genus and other deep‐sea bacteria, thus, could be used as a versatile reference gene for RT‐qPCR analysis of these microorganisms under extreme environmental conditions.
Validation of reference genes for reverse transcription real-time quantitative PCR analysis in the deep-sea bacterium Shewanella psychrophila WP2.
TLDR
The rho gene is conserved in Shewanella genus and other deep-sea bacteria, thus, could be used as a versatile reference gene for RT-qPCR analysis of these microorganisms under extreme environmental conditions.
The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes
TLDR
The study reveals putative evolutionary scenario of the dnd system and provides novel insights into the physiological and ecological influences of PT modification, which suggests that a series of negative effects on microorganisms caused by dnd systems limit horizontal gene transfer, thus leading to their sporadic distribution.