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This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher(More)
The activation of hepatic stellate cells (HSCs) is involved in the development of hepatic fibrosis. Previous studies have indicated that the acquisition of certain properties by activated HSCs is highly dependent on the reorganization of the actin cytoskeleton. However, direct evidence showing that the reorganization of the actin cytoskeleton is responsible(More)
Ulvan extracted from Ulva pertusa (Chlorophyta) is a group of sulfated heteropolysaccharide, for simplicity, the sulfated polysaccharide is referred to as ulvan in this paper. To our knowledge, there is no detailed report investigating the toxicity of ulvan. In this study, the subchronic (6 months) toxicity of varying levels of ulvan extracted from U.(More)
In the present paper, the genotoxicity of transgenic Synechocystis sp. PCC 6803 containing flounder (Paralichthys olivaceus) growth hormone gene has been assessed using micronucleus tests in mice and fish. No micronucleus inductions in bone marrow erythrocyte of mice were observed at three doses of transgenic Synechocystis (2.0, 5.0, and 10.0g/kg). The(More)
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