Shuichiro Fukushima

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The effects of herbicides on algal production in the Kokai River were assessed using a Selenastrum growth inhibition test and by an investigation of the periphyton formed in a channel carrying river water. Herbicides in the water were analysed at the same intervals as the algal growth test (three times per week) from April to August 1992. Growth of(More)
In chronic kidney disease (CKD), osteodystrophy and arterial calcification often coexist. However, arterial alterations have not been addressed in CKD unaccompanied by evidence of calcification. We investigated the association of phosphate (P) and calcium (Ca) accumulation in calcification-free aortas with CKD-induced osteodystrophy. Aortic accumulation of(More)
The purified bacteriocin was sensitive to proteolytic enzymes such as trypsin, alpha-chymotrypsin and pronase, but resistant to papain and pepsin. Lowering the pH of the bacteriocin caused precipitation, and the Abs280 of the supernatant reached a minimum at pH 3.6, suggesting that this is the isoelectric point. Such a pH value coincides with minimum(More)
Cross-linking of collagen by Advanced Glycation End-products (AGEs) occurs by non-enzymatic glycation (Maillard reaction). The purpose of this study was to examine whether AGEs are formed in human dentinal collagen, and to consider any possible influence of AGEs on dentinal physiology. Mechanical characteristics, fluorescence spectra and immunohistochemical(More)
Vascular calcification is commonly associated with aging. Quantification of calcium accumulation in vessel walls is important in understanding the mechanisms of vascular calcification. To elucidate age-related change of calcification, site dependence of calcification, and the effect of hemodynamic stress on calcification, we measured calcium contents in(More)
Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A(More)
Second-harmonic-generation (SHG) microscopy is an interesting new tool for observing dermal collagen fiber in skin. However, conventional SHG microscopy using a mode-locked Ti:sapphire laser suffers from low penetration depth and a slow image acquisition rate caused by scattering and absorption in tissue, making it difficult to use for in vivo applications(More)
Polarization-resolved second-harmonic-generation (PR-SHG) microscopy is a powerful tool for investigating collagen fiber orientation quantitatively with low invasiveness. However, the waiting time for the mechanical polarization rotation makes it too sensitive to motion artifacts and hence has hampered its use in various applications in vivo. In the work(More)