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The response of Leptospirillum ferriphilum ML-04 to arsenic stress was analyzed using two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Thirty-eight of 65 significantly differentially expressed arsenic response proteins were identified, and 25 of them have known functions.(More)
Leptospirillum ferriphilum has been identified as the dominant, moderately thermophilic, bioleaching microorganism in bioleaching processes. It is an acidic and chemolithoautrophic bacterium that gains electrons from ferrous iron oxidation for energy production and cell growth. Genetic information about this microorganism has been limited until now, which(More)
A wide-host-range plasmid pTRUS containing a homologous rus gene under the control of Ptac promoter was constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer method to generate the engineered strain of A. ferrooxidans(pTRUS). The plasmid-based recombinant rus gene was successfully expressed. According to(More)
Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe(3+) ) by oxidization of ferrous iron (Fe(2+) ). The high-molecular-weight c-type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe(2+) oxidation respiratory pathway of A.(More)
ChinaGrid provides the nationwide grid computing platform and services for education and research purpose among 100 key universities in China. University digital museum grid (UDMGrid) is one of the most important applications of ChinaGrid which aims at offering one-stop information services about kinds of digital specimens from several university digital(More)
BACKGROUND Pedicle screw misplacement rates are relatively high with fluoroscopically guided techniques. However, breach rates in the 5th lumbar and the 1st sacral spines in conventional operations have not been specifically concerned because of their broad cross sections. It's a retrospective study to evaluate the accuracy and safety of pedicle screw(More)
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