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A truncated Fibrobacter succinogenes 1,3-1,4-beta-d-glucanase with improved enzymatic activity and thermotolerance.
As an approach to improving Fibrobacter succinogenes 1,3-1,4-beta-d-glucanase (Fsbeta-glucanase) for use in industry and to studying the structure-function relationship of the C-terminus in theExpand
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Mutagenesis of Trp(54) and Trp(203) residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalytic activities of the enzyme.
The possible structural and catalytic functions of the nine tryptophan amino acid residues, including Trp(54), Trp(105), Trp(112), Trp(141), Trp(148), Trp(165), Trp(186), Trp(198), and Trp(203) inExpand
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Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose.
Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) catalyzes the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans or lichenan. ThisExpand
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Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes.
The 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes (Fsbeta-glucanase) is classified as one of the family 16 glycosyl hydrolases. It hydrolyzes the glycosidic bond in the mixed-linked glucansExpand
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Molecular and immunological characterization and IgE epitope mapping of Pen n 18, a major allergen of Penicillium notatum.
The mould genus, Penicillium, is a significant source of environmental aero-allergens. A major allergen from Penicillium notatum, Pen n 18, was identified by two-dimensional immunoblotting usingExpand
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Structural and catalytic roles of residues located in β13 strand and the following β-turn loop in Fibrobacter succinogenes 1,3-1,4-β-d-glucanase
Abstract Background Fibrobacter succinogenes 1,3-1,4-β- d -glucanase (Fsβ-glucanase) is the only naturally occurring circularly permuted β-glucanase among bacterial glucanases with reverse proteinExpand
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Mutational and structural studies of the active-site residues in truncated Fibrobacter succinogenes1,3-1,4-beta-D-glucanase.
1,3-1,4-beta-D-Glucanases (EC specifically hydrolyze beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in lichenan or beta-D-glucans. It has been suggested thatExpand
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Different strains of Acinetobacter baumannii spreading in an intensive care unit
Abstract Background An outbreak of Acinetobacter baumannii in neurological intensive care unit was detected, causing nosocomial infection on 6 cases and colonization on 5 cases. Methods Five majorExpand
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P 1003 Crystal Structure of FlgD from Xanthomonas campestris : Insights into the Hook Capping Essential for Flagellar Assembly
Ming-Tao Lee , Wei-Chin Hung, Fang-Yu Chen and Huey W. Huang National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan Department of Physics, Chinese Military Academy, Fengshan,Expand