Shu Wei

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The Arabidopsis microRNA156 (miR156) regulates 11 members of the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) family by base pairing to complementary target mRNAs. Each SPL gene further regulates a set of other genes; thus, miR156 controls numerous genes through a complex gene regulation network. Increased axillary branching occurs in transgenic Arabidopsis(More)
The porcine pluripotent cells that can generate germline chimeras have not been developed. The Oct4 promoter-based fluorescent reporter system, which can be used to monitor pluripotency, is an important tool to generate authentic porcine pluripotent cells. In this study, we established a porcine Oct4 reporter system, wherein the endogenous Oct4 promoter(More)
Tea plants (Camellia sinensis) are used to produce one of the most important beverages worldwide. The nutritional value and healthful properties of tea are closely related to the large amounts of three major characteristic constituents including polyphenols (mainly catechins), theanine and caffeine. Although oil tea (Camellia oleifera) belongs to the genus(More)
In this study, recalcitrance of tea plant ( Camellia sinensis) to Agrobacterium-mediated genetic transformation was investigated with an emphasis on specialized compounds in tea. Chemical constituents in tea leaves and calli were extracted into liquid Luria–Bertani (LB) medium to determine their biological activities on Agrobacterium growth, virulence, and(More)
Epigenetic regulation of gene expression is critical for controlling embryonic properties during the embryo-to-seedling phase transition. Here we report that a histone deacetylase19 (HDA19)-associated regulator, scarecrow-like15 (SCL15), is essential for repressing the seed maturation programme in vegetative tissues. SCL15 is expressed in and GFP-tagged(More)
The recently emerged CRISPR/Cas9 technique has opened a new perspective on readily editing specific genes. When combined with transcription activators, it can precisely manipulate endogenous gene expression. Here, we enhanced the expression of endogenous Cdx2 and Gata6 genes by CRISPR-mediated activators, thus mouse embryonic stem cells (ESCs) were directly(More)
Complex T-DNA repeat structures are a frequent outcome of Agrobacterium-mediated plant transformation and often lead to co-suppression of gene expression, even gene silencing. Thus, they are undesired in transgenic plants for commercial or research purposes and frequently need to be efficiently identified in a large transgenic plant population. Application(More)
INTRODUCTION Successful percutaneous endoscopic lumbar discectomy (PELD) requires an appropriate working trajectory. Due to the complexity of spinal anatomy, this is difficult to verify with conventional 2-dimensional fluoroscopy. AIM Here we assessed the feasibility and utility of the O-arm for establishing the working trajectory for PELD. MATERIAL AND(More)
Transgenic Nicotiana tabacum plants expressing Aspergillus niger b-glucosidase (EC 3.2.1.21) gene (BGL1) in different subcellular compartments [cell wall (Tcw), endoplasmic reticulum (Ter), and vacuole (Tvc)] were analyzed to study the effects of BGL1 localization on plant growth and plant–insect interaction. Transgenic and non-transgenic plants were grown(More)
A broadband metamaterial (MM) absorber based on water droplets is proposed and investigated in this paper. The proposed MM absorber is composed of a periodic array of holes drilled in the dielectric substrate which are then filled with water droplets. The absorption property of such water absorber can be tuned by manipulating the volume of the water(More)