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A quantitative one-step SYBR Green I-based reverse transcription (RT)-PCR system was developed for the detection and differentiation of four different dengue virus serotypes in acute-phase serum samples. A set of group- and serotype-specific primer pairs was designed against conserved sequences in the core region and evaluated for clinical diagnosis. A(More)
Airport fever screening in Taiwan, July 2003-June 2004, identified 40 confirmed dengue cases. Results obtained by capture immunoglobulin (Ig) M and IgG enzyme-linked immunoassay, real time 1-step polymerase chain reaction, and virus isolation showed that 33 (82.5%) of 40 patients were viremic. Airport fever screening can thus quickly identify imported(More)
Europe (8), resident populations in these countries have been exposed to these virus lineages more frequently than populations in Asia, and therefore may have acquired a greater degree of preexisting cross-reactive immunity to pandemic (H1N1) 2009 virus. A recent review of human swine infl uenza infections suggests that they may not be uncommon (9),(More)
Japanese encephalitis (JE) is a mosquito-borne zoonotic disease caused by the Japanese encephalitis virus (JEV). Pigs and water birds are the main amplifying and maintenance hosts of the virus. In this study, we conducted a JEV survey in mosquitoes captured in pig farms and water bird wetland habitats in Taiwan during 2005 to 2012. A total of 102,633(More)
tosporidium genotypes in sporadic cryp-tosporidiosis cases: fi rst report of human infections with a cervine genotype. A population genetic study of the Cryptosporidium parvum human genotype parasites. Genotype and subtype analyses of Cryp-tosporidium isolates from dairy calves and humans in Ontario. L, Sulaiman I, et al. Identifi cation of two different(More)
We identified 78 imported chikungunya cases in Taiwan during 2006-2014. Sixty-six (84.6%) cases were initially suspected to be dengue, which indicates the necessity for laboratory diagnostics in differentiation between dengue and chikungunya. Results also emphasize the need for active surveillance of febrile illness at points of entry.
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