Shree Prasad Thapa

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Soil is the major sink for majority of pesticides applied on agricultural crops and its fate depends on variety of factors. There is little research on fate of pesticide in field soil under different climatic conditions and there is a need of study on the influence of climate on pesticide degradation and persistence in soil. In the present study, the(More)
Screening of wild tomato accessions revealed a source of resistance to Pseudomonas syringe pv. tomato race 1 from Solanum habrochaites and facilitated mapping of QTLs controlling disease resistance. Pseudomonas syringae pv. tomato (Pst) causes bacterial speck of tomato, which is one of the most persistent bacterial diseases in tomato worldwide. Existing Pst(More)
We developed a multiplex PCR detection assay for quick diagnosis of Pantoea stewartii subsp. stewartii the causal agent of Stewart’s bacterial wilt and leaf blight of maize, using specific genetic markers. The genetic markers of wtsE, cpsA and hrpN gene specifically amplified 519 bp, 375 bp and 200 bp of DNA, respectively, from all isolates of P. stewartii(More)
Japanese Erwinia pyrifoliae strains cause bacterial shoot blight of pear (BSBP) in Japan. The genetics of Japanese Erwinia remains largely unknown relative to the abundant genomic information available for other Erwinia strains. We compared the genome of Japanese and Korean E. pyrifoliae strains along with those of E. amylovora and E. tasmaniensis.(More)
Clavibacter michiganensis subsp. michiganensis is a gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing bacterial canker disease. In this study, we sequenced and assembled genomes of 11 C. michiganensis subsp. michiganensis strains isolated from infected tomato fields in California as well as five Clavibacter strains(More)
In 1995, a serious necrotic disease appeared in Asian pear trees in the orchards of Chuncheon, South Korea. Large numbers of bacterial samples were collected, and the causative microorganism was identified as a novel pathogen, Erwinia pyrifoliae. Among the samples, four bacterial isolates with atypical characteristics were further analyzed through(More)
Citation: Pagliaccia D, Shi J, Pang Z, Hawara E, Clark K, Thapa SP, Francesco AD, Liu J, Tran T-T, Bodaghi S, Folimonova SY, Ancona V, Mulchandani A, Coaker G, Wang N, Vidalakis G and Ma W (2017) A Pathogen Secreted Protein as a Detection Marker for Citrus Huanglongbing. Front. Microbiol. 8:2041. doi: 10.3389/fmicb.2017.02041 A Pathogen Secreted Protein as(More)
A multiplex PCR and DNA array for quick detection of Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. was developed using specific genetic markers derived from virulence-related genes. The genetic markers of cytK, sei, prfA, rfB, and hilA gene specifically amplified DNA fragments of 320 bp, 500(More)
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